beta Catenin antibody (CF®488A)

Catalog No. ABIN6183668

This anti-beta Catenin antibody is a Rabbit Polyclonal antibody detecting beta Catenin in WB, FACS, IF and IHC (f). Suitable for Human and Mouse.

Quick Overview for beta Catenin antibody (CF®488A) (ABIN6183668)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Catenin antibody is conjugated to CF®488A

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: PAb

Product Details anti-beta Catenin Antibody (CF®488A)

Purpose: Rabbit Polyclonal anti-Beta-Catenin (p120), CF488A Conjugate

Characteristics: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide from the middle of beta-Catenin (p120) protein

Isotype: IgG kappa

Application Details

Application Notes: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa or MCF-7 cells. Breast carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: Beta-Catenin

Background: Cadherin associated protein), beta 1 88  kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1

Molecular Weight: 92 kDa

Gene ID: 1499, 476018

UniProt: P35222

Pathways: Peptide Hormone Metabolism